Change search
ReferencesLink to record
Permanent link

Direct link
The influence of Ceratocystis polonica inoculation and methyl jasmonate application on terpene chemistry of Norway spruce, Picea abies
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
Show others and affiliations
2010 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 71, no 11-12, 1332-1341 p.Article in journal (Refereed) Published
Abstract [en]

Constitutive and inducible terpene production is involved in conifer resistance against bark beetles and their associated fungi. In this study 72 Norway spruce (Picea abies) were randomly assigned to methyl jasmonate (MJ) application, inoculation with the bluestain fungus Ceratocystis polonica, or no-treatment control. We investigated terpene levels in the stem bark of the trees before treatment, 30 days and one year after treatment using GC-MS and two-dimensional GC (2D-GC) with a chiral column, and monitored landing and attack rates of the spruce bark beetle, Ips typographus, on the trees by sticky traps and visual inspection. Thirty days after fungal inoculation the absolute amount and relative proportion of (+)-3-carene, sabinene, and terpinolene increased and (+)-alpha-pinene decreased. Spraying the stems with MJ tended to generally increase the concentration of most major terpenes with minor alteration to their relative proportions, but significant increases were only observed for (-)-beta-pinene and (-)-limonene. Fungal inoculation significantly increased the enantiomeric ratio of (-)-alpha-pinene and (-)-limonene 1 month after treatment, whereas MJ only increased that of (-)-limonene. One year after treatment, both MJ and fungal inoculation increased the concentration of most terpenes relative to undisturbed control trees, with significant changes in (-)-beta-pinene, (-)-beta-phellandrene and some other compounds. Terpene levels did not change in untreated stem sections after treatment, and chemical induction by MJ and C polonica thus seemed to be restricted to the treated stem section. The enantiomeric ratio of (-)-alpha-pinene was significantly higher and the relative proportions of ( -)-limonene were significantly lower in trees that were attractive to bark beetles compared to unattractive trees. One month after fungal inoculation, the total amount of diterpenes was significantly higher in putative resistant trees with shorter lesion lengths than in putative susceptible trees with longer lesions. Thus, terpene composition in the stem bark may be related to resistance of Norway spruce against I. typographus and C. polonica.

Place, publisher, year, edition, pages
2010. Vol. 71, no 11-12, 1332-1341 p.
Keyword [en]
Picea abies, Ceratocystis polonica, Methyl jasmonate, Terpene, Defense, Ips typographus
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-29456DOI: 10.1016/j.phytochem.2010.05.017ISI: 000280321000015ScopusID: 2-s2.0-77954244208OAI: diva2:396001
QC 20110208Available from: 2011-02-08 Created: 2011-02-02 Last updated: 2011-05-03Bibliographically approved
In thesis
1. Conifer chemical defense: Rugulation of bark beetle colonization and pheromone emission
Open this publication in new window or tab >>Conifer chemical defense: Rugulation of bark beetle colonization and pheromone emission
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Terpenes and phenols are of importance in conifer defense against insects and pathogens. Knowledge about tree chemical defense is vital for developing practical methods to maintain healthy forests. With the aims of characterizing the defensive chemical induction in Norway spruce Picea abies and demonstrating its ecological function to spruce bark beetle Ips typographus, we measured the terpenoid and phenolic content in the bark of mature Norway spruce trees suffering windstorm, inoculated with Ceratocystis polonica, or treated with methyl jasmonate (MeJA), and investigated the colonization and pheromone emission of I. typographus.

All three stressors altered the chemical profile in the bark of Norway spruce. Trees damaged by windstorm had lower proportions of (+)-3-carene and two unidentified stilbenes, and a higher taxifolin glycoside content than trees without apparent windstorm damage; C. polonica inoculation induced extremely strong quantitative terpene accumulation in the wound reaction zone, but only increased the levels of (+)-3-carene, sabinene and terpinolene in the bark near the reaction zone; MeJA treatment generally elicited quantitative terpene accumulation, but the induction differed extensively between individual trees. In addition, logs from MeJA-treated tree showed much stronger wounding response compared to control logs.

The chemical profile of Norway spruce affected the colonization and pheromone emission of I. typographus. In response to fungal inoculation, terpene present in the reaction zone inhibited the colonization of I. typographus in a dose-dependent manner. Host defense elicited by MeJA treatment reduced emissions of 2-methyl-3-buten-2-ol and (S)-cis-verbenol, the two key aggregation pheromone components of I. typographus, and altered the ratio between the two components.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2011. 75 p.
Trita-CHE-Report, ISSN 1654-1081 ; 2011:16
Picea abies, Ips typographus, Ceratocystis polonica, methyl jasmonate, windstorm, stress response, terpenes, phenols, colonization, pheromone emission
National Category
Agricultural Science
urn:nbn:se:kth:diva-33199 (URN)978-91-7415-884-7 (ISBN)
Public defence
2011-05-23, sal F3, Lindstedtsvägen 26, KTH, Stockholm, 15:52 (English)
QC 20110503Available from: 2011-05-03 Created: 2011-04-29 Last updated: 2011-05-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Zhao, TaoBorg-Karlson, Anna-Karin
By organisation
Organic Chemistry
In the same journal
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 58 hits
ReferencesLink to record
Permanent link

Direct link