A Homogeneous Assay for Protein Analysis in Droplets by Fluorescence Polarization
2012 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 33, no 3, 436-439 p.Article in journal (Refereed) Published
We present a novel homogeneous (mix-incubate-read) droplet microfluidic assay for specific protein detection in picoliter volumes by fluorescence polarization (FP), for the first time demonstrating the use of FP in a droplet microfluidic assay. Using an FP-based assay we detect streptavidin concentrations as low as 500?nM and demonstrate that an FP assay allows us to distinguish droplets containing 5?mu M rabbit IgG from droplets without IgG with an accuracy of 95%, levels relevant for hybridoma screening. This adds to the repertoire of droplet assay techniques a direct protein detection method which can be performed entirely inside droplets without the need for labeling of the analyte molecules.
Place, publisher, year, edition, pages
2012. Vol. 33, no 3, 436-439 p.
Droplet microfluidics, Fluorescence polarization, Homogeneous assays, Miniaturization, Proteins
IdentifiersURN: urn:nbn:se:kth:diva-30459DOI: 10.1002/elps.201100350ISI: 000299629800004ScopusID: 2-s2.0-84863075291OAI: oai:DiVA.org:kth-30459DiVA: diva2:400225
FunderSwedish Research CouncilKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 20110225. Updated from manuscript to article in journal.2011-02-252011-02-252013-04-15Bibliographically approved