A hierarchical classification of polysaccharide lyases for glycogenomics
2010 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 432, 437-444 p.Article in journal (Refereed) Published
Carbohydrate-active enzymes face huge substrate diversity in a highly selective manner using only a limited number of available folds. They are therefore subjected to multiple divergent and convergent evolutionary events. This and their frequent modularity render their functional annotation in genomes difficult in a number of cases. In the present paper, a classification of polysaccharide lyases (the enzymes that cleave polysaccharides using an elimination instead of a hydrolytic mechanism) is shown thoroughly for the first time. Based on the analysis of a large panel of experimentally characterized polysaccharide lyases, we examined the correlation of various enzyme properties with the three levels of the classification: fold, family and subfamily. The resulting hierarchical classification, which should help annotate relevant genes in genomic efforts, is available and constantly updated at the Carbohydrate-Active Enzymes Database (http://www.cazy.org).
Place, publisher, year, edition, pages
2010. Vol. 432, 437-444 p.
catalytic mechanism, enzyme family, functional annotation, modular structure, polysaccharide lyase
IdentifiersURN: urn:nbn:se:kth:diva-30566DOI: 10.1042/BJ20101185ISI: 000285664700003ScopusID: 2-s2.0-78649868163OAI: oai:DiVA.org:kth-30566DiVA: diva2:401003
FunderFormasSwedish Research Council
QC 201103012011-03-012011-02-282014-09-24Bibliographically approved