In Situ Evaluation of Lipase Performances Through Dynamic Asymmetric Cyanohydrin Resolution
2011 (English)In: Organic and biomolecular chemistry, ISSN 1477-0520, E-ISSN 1477-0539, Vol. 9, no 4, 1112-1117 p.Article in journal (Refereed) Published
A dynamic resolution process based on multiple reversible cyanohydrin formation coupled to lipase-mediated transesterification is demonstrated. The resulting process resulted in the efficient evaluation of complex lipase performances in asymmetric cyanohydrin acylate synthesis. Dynamic systems were generated and resolved in situ, and the effects of the reaction conditions could be directly monitored for the overall system. By this concept, the enzyme activity, chemo- and stereoselectivity for all involved substrates could be simultaneously evaluated.
Place, publisher, year, edition, pages
2011. Vol. 9, no 4, 1112-1117 p.
IdentifiersURN: urn:nbn:se:kth:diva-30515DOI: 10.1039/c0ob00759eISI: 000286895900023ScopusID: 2-s2.0-79551703938OAI: oai:DiVA.org:kth-30515DiVA: diva2:404018
FunderSwedish Research CouncilEU, European Research Council, MRTN-CT-2006-035614
QC 201103152011-03-152011-02-282011-03-15Bibliographically approved