Covalent VEGF protein immobilization on resorbable polymeric surfaces
2011 (English)In: POLYMERS FOR ADVANCED TECHNOLOGIES , 2011, Vol. 22, no 1, 166-171 p.Conference paper (Refereed)
Vascular endothelial growth factor (VEGF) type protein, a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L-lactic acid) (PLLA) and poly(epsilon-caprolactone) through a three-step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non-destructive and solvent-free vapor-phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride/N-hydroxysuccinimide chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with X-ray photoelectron spectroscopy, attenuated total reflectance-Fourier transform infrared spectrometry, UV-VIS, scanning electron microscopy, and enzyme-linked immunosorbent assay.
Place, publisher, year, edition, pages
2011. Vol. 22, no 1, 166-171 p.
, Polymers for advanced technologies, ISSN 1042-7147
polylactide, poly(epsilon-caprolactone), vapor-phase grafting, VEGF, surface
IdentifiersURN: urn:nbn:se:kth:diva-30507DOI: 10.1002/pat.1811ISI: 000285881800024OAI: oai:DiVA.org:kth-30507DiVA: diva2:404364
10th International Conference on Polymers for Advanced Technologies (PAT 2009)
QC 201103162011-03-162011-02-282011-03-16Bibliographically approved