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Comparative proteomic profiles of the marine cyanobacterium Trichodesmium erythraeum IMS101 under different nitrogen regimes
KTH, School of Biotechnology (BIO), Glycoscience.
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2011 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 11, no 3, 406-419 p.Article in journal (Refereed) Published
Abstract [en]

Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and subtropical oceans. It is unique in that it exclusively fixes N-2 at daytime, although it belongs to the non-heterocystous filamentous segment of the cyanobacterial radiation. Here we present the first quantitative proteomic analysis of Trichodesmium erythraeum IMS101 when grown under different nitrogen regimes using 2-DE/MALDI-TOF-MS. Addition of combined nitrogen (NO3-) prevented development of the morphological characteristics of the N-2-fixing cell type (diazocytes), inhibited expression of the nitrogenase enzyme subunits and consequently N-2 fixation activity. The diazotrophic regime (N-2 versus NO3- cultures) elicited the differential expression of more than 100 proteins, which represented 13.5% of the separated proteins. Besides proteins directly related to N-2 fixation, proteins involved in the synthesis of reducing equivalents and the generation of a micro-oxic environment were strongly up-regulated, as was in particular Dps, a protein related to iron acquisition and potentially other vital cellular processes. In contrast, proteins involved in the S-adenosylmethionine (SAM) cycle, synthesis of amino acids and production of carbon skeletons for storage and synthesis of amino acids were suppressed. The data are discussed in the context of Trichodesmium's unusual N-2-fixing physiology.

Place, publisher, year, edition, pages
2011. Vol. 11, no 3, 406-419 p.
Keyword [en]
Combined nitrogen, Diazocytes, Marine cyanobacteria, Microbiology, Nitrogen fixation
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-31302DOI: 10.1002/pmic.201000382ISI: 000287718000007ScopusID: 2-s2.0-79251529884OAI: diva2:404916
Swedish Research CouncilKnut and Alice Wallenberg Foundation
QC 20110318Available from: 2011-03-18 Created: 2011-03-14 Last updated: 2011-03-18Bibliographically approved

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