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Generation and validation of affinity reagents on a proteome-wide level
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-8993-048X
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0003-0605-8417
2009 (English)In: Journal of Molecular Recognition, ISSN 0952-3499, E-ISSN 1099-1352, Vol. 22, no 2, 57-64 p.Article in journal (Refereed) Published
Abstract [en]

There is a need for protein-specific affinity reagents to explore the gene products encoded by the genome. Recently, systematic efforts to generate validated affinity reagents on a whole human proteome level have been initiated. There are several issues for such efforts, including choice of antigen, type of affinity reagent, and the subsequent validation of the generated protein-specific binders. The advantages and disadvantages with the different approaches are discussed and the problems related to quality assessment of antibodies to be used in multi-platform applications are addressed. This review also describes the efforts to create a virtual resource of validated antibodies using a community-based portal and summarizes the status and visions for the publicly available human protein atlas (www.proteinatlas.org) showing the human protein profiles in a large number of normal and cancer tissues as well as a large set of human cell lines.

Place, publisher, year, edition, pages
2009. Vol. 22, no 2, 57-64 p.
Keyword [en]
affinity reagents, validation, antigens, human protein atlas
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-31257DOI: 10.1002/jmr.891ISI: 000263566400002Scopus ID: 2-s2.0-63449121086OAI: oai:DiVA.org:kth-31257DiVA: diva2:405529
Note
QC 20110322Available from: 2011-03-22 Created: 2011-03-11 Last updated: 2017-12-11Bibliographically approved

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Uhlén, MathiasHober, Sophia

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