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Automated unrestricted multigene recombineering for multiprotein complex production
KTH, School of Engineering Sciences (SCI), Applied Physics.
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2009 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 6, no 6, 447-U68 p.Article in journal (Refereed) Published
Abstract [en]

Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.

Place, publisher, year, edition, pages
2009. Vol. 6, no 6, 447-U68 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-32150DOI: 10.1038/NMETH.1326ISI: 000266493300017Scopus ID: 2-s2.0-67349278136OAI: oai:DiVA.org:kth-32150DiVA: diva2:409245
Funder
EU, FP7, Seventh Framework Programme
Note
QC 20110407Available from: 2011-04-07 Created: 2011-04-07 Last updated: 2017-12-11Bibliographically approved

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