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Molecular cryo-electron tomography of vitreous tissue sections: current challenges
KTH, School of Engineering Sciences (SCI), Centres, Center for Industrial and Applied Mathematics, CIAM. KTH, School of Engineering Sciences (SCI), Mathematics (Dept.).ORCID iD: 0000-0002-1118-6483
2009 (English)In: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, Vol. 235, no 3, 293-307 p.Article in journal (Refereed) Published
Abstract [en]

Electron tomography of vitreous tissue sections (tissue TOVIS) allows the study of the three-dimensional structure of molecular complexes in a near-native cellular context. Its usage is, however, limited by an unfortunate combination of noisy and incomplete data, by a technically demanding sample preparation procedure, and by a disposition for specimen degradation during data collection. Here we outline some major challenges as experienced from the application of TOVIS to human skin. We further consider a number of practical measures as well as theoretical approaches for its future development.

Place, publisher, year, edition, pages
2009. Vol. 235, no 3, 293-307 p.
Keyword [en]
CEMOVIS, electron tomography, regularization, skin, tomography of vitreous sections, TOVIS
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-32555DOI: 10.1111/j.1365-2818.2009.03219.xISI: 000269187500007Scopus ID: 2-s2.0-69249119907OAI: oai:DiVA.org:kth-32555DiVA: diva2:411062
Note
QC 20110415Available from: 2011-04-15 Created: 2011-04-15 Last updated: 2017-12-11Bibliographically approved

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Öktem, Ozan

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