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A comparison between protein profiles of B cell subpopulations and mantle cell lymphoma cells
KTH, School of Biotechnology (BIO), Gene Technology.
2009 (English)In: Proteome Science, ISSN 1477-5956, E-ISSN 1477-5956, Vol. 7, 43- p.Article in journal (Refereed) Published
Abstract [en]

Background: B-cell lymphomas are thought to reflect different stages of B-cell maturation. Based on cytogenetics and molecular markers, mantle cell lymphoma (MCL) is presumed to derive predominantly from naive, pre-germinal centre (pre-GC) B lymphocytes. The aim of this study was to develop a method to investigate the similarity between MCL cells and different B-cell compartments on a protein expression level. Methods: Subpopulations of B cells representing the germinal centre (GC), the pre-GC mantle zone and the post-GC marginal zone were isolated from tonsils using automated magnetic cell sorting (AutoMACS) of cells based on their expression of CD27 and IgD. Protein profiling of the B cell subsets, of cell lines representing different lymphomas and of primary MCL samples was performed using top-down proteomics profiling by surface-enhanced laser detection/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Results: Quantitative MS data of significant protein peaks (p-value < 0.05) separating the three B-cell subpopulations were generated. Together, hierarchical clustering and principal component analysis (PCA) showed that the primary MCL samples clustered together with the pre- and post-GC subpopulations. Both primary MCL cells and MCL cell lines were clearly separated from the B cells representing the GC compartment. Conclusion: AutoMACS sorting generates sufficient purity to enable a comparison between protein profiles of B cell subpopulations and malignant B lymphocytes applying SELDI-TOF-MS. Further validation with an increased number of patient samples and identification of differentially expressed proteins would enable a search for possible treatment targets that are expressed during the early development of MCL.

Place, publisher, year, edition, pages
2009. Vol. 7, 43- p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:kth:diva-32704DOI: 10.1186/1477-5956-7-43ISI: 000272709900001Scopus ID: 2-s2.0-71949127588OAI: oai:DiVA.org:kth-32704DiVA: diva2:411753
Funder
EU, FP7, Seventh Framework Programme
Note
QC 20110419Available from: 2011-04-19 Created: 2011-04-18 Last updated: 2017-12-11Bibliographically approved

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