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CASCAT: Redesign of omega-Transaminases for Synthesis of Chiral Amines
KTH, School of Biotechnology (BIO), Biochemistry (closed 20130101).ORCID iD: 0000-0003-2371-8755
KTH, School of Biotechnology (BIO), Biochemistry (closed 20130101).
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2010 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 150, S123-S124 p.Article in journal (Other academic) Published
Abstract [en]

Transaminases (EC are attractive biocatalysts for synthesis of chiral amines and alpha-amino acids. These enzymes catalyze transfer of an amine group from a donor substrate to an acceptor compound using the cofactor pyridoxal-5′-phoshate (PLP). omega-Transaminases are a versatile subgroup of the transaminases that does not require a carboxylic acid group in alpha-position (in contradiction toalpha-transaminases) and hence accept a wider spectrum of ketones or amines. The omega-transaminases are employed industrially for production of both R- and S-enantiopure amines.

One bottleneck is the unfavourable equilibrium in such reactions run in the synthesis mode. We have developed a one-pot multi-enzyme system in a cascade fashion for equilibrium displacement by removing formed acetone.

Another issue is the fact that most omega-transaminases show S-selectivity, however a few R-selective strains do exist. We have used an S-selective omega-transaminase variant from Arthrobacter citreus and created an R-selective variant by rational redesign using a homology enzyme model. This homology modelling/rational design approach was further explored on an omega-transaminase from Chromobacterium violaceum.

Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 150, S123-S124 p.
Keyword [en]
Biocatalysis, Enzymology, Chiral amine, Transamination
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-33225DOI: 10.1016/j.jbiotec.2010.08.321ISI: 000288873400303OAI: diva2:416511
14th International Biotechnology Symposium and Exhibition (IBS-2008) Rimini, ITALY SEP 14-18, 2010

QC 20110512

Available from: 2011-05-12 Created: 2011-05-02 Last updated: 2016-04-18Bibliographically approved

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Svedendahl, MariaEngelmark Cassimjee, KarimBerglund, Per
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