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3D local structure around Zn in Kti11p as a representative Zn-(Cys)4 motif as obtained by MXAN
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2008 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 374, no 1, 28-32 p.Article in journal (Refereed) Published
Abstract [en]

Zinc is an important component of many proteins that play key roles in transcription, translation, and catalysis. Kti11p, DESR1, both belonging to a protein family characterized by a CSL zinc finger domain, and the co-catalytic zinc-protein PML containing a Zn2+ binding domain called RING or C3HC4 finger are all structurally determined by NMR although the zinc sites are silent to this spectroscopical method. The comparison of X-ray absorption near-edge spectroscopy (XANES) data for the three proteins demonstrates that fingerprints effect is a reliable method for a primary characterization of ligand species. Ab initio full MS Calculations performed by MAN are applied to obtain chemical and stereo structural information around the Zn ion in Kti11p. For the first time this high-spatial resolution technique confirms the formation of a stable Zn tetrahedral configuration with four sulfur ligands, and returns extremely accurate bond angle information between ligands.

Place, publisher, year, edition, pages
2008. Vol. 374, no 1, 28-32 p.
Keyword [en]
zinc motif, Kti11p, EXAFS, XANES, fingerprints, MXAN
National Category
Industrial Biotechnology
URN: urn:nbn:se:kth:diva-34300DOI: 10.1016/j.bbrc.2008.06.116ISI: 000258798700006ScopusID: 2-s2.0-47949093589OAI: diva2:420477
QC 20110601Available from: 2011-06-01 Created: 2011-06-01 Last updated: 2011-06-01Bibliographically approved

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Gao, Bin
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Theoretical Chemistry (closed 20110512)
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