Bifunctional Dendronized Cellulose Surfaces as Biosensors
2011 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 12, no 6, 2114-2125 p.Article in journal (Refereed) Published
Well-defined dendronized cellulose substrates displaying multiple representations of dual-functionality were constructed by taking advantage of the efficiency of the click reaction combined with traditional anhydride chemistry. First, activated cellulose surfaces were decorated with several generations of dendrons, and their peripheral reactive groups were subsequently reacted with a trifunctional orthogonal monomer. The generated substrate tool box was successfully explored by accurately tuning the surface function using a versatile orthogonal dual postfunctionalization approach. In general, the reactions were monitored by using a click-dye reagent or a quartz crystal microbalance (QCM) technique, and the resulting surfaces were well-characterized using XPS, and contact angle measurements. Utilizing this approach two different surfaces have been obtained; that is, triethylenglycol oligomers and amoxicillin molecules were efficiently introduced to the dendritic surface. As a second example, mannose-decorated hydroxyl functional surfaces illustrated their potential as biosensors by multivalent detection of lectin protein at concentration as low as 5 nM.
Place, publisher, year, edition, pages
2011. Vol. 12, no 6, 2114-2125 p.
CLICK-CHEMISTRY, EFFICIENT SYNTHESIS, FUTURE-DIRECTIONS, DENDRIMERS, AZIDE, FILMS
IdentifiersURN: urn:nbn:se:kth:diva-35633DOI: 10.1021/bm200201yISI: 000291499900019ScopusID: 2-s2.0-79958779836OAI: oai:DiVA.org:kth-35633DiVA: diva2:429232
FunderSwedish Research Council, 2006-3617Swedish Research Council, 2009-3259
QC 201107042011-07-042011-07-042013-08-30Bibliographically approved