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Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides
KTH, School of Biotechnology (BIO).
2007 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 71, no 1, 87-89 p.Article in journal (Refereed) Published
Abstract [en]

BioRad's Rotofor (R) system has been frequently used for the purification of proteins and smaller pepticles such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor (R) isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

Place, publisher, year, edition, pages
2007. Vol. 71, no 1, 87-89 p.
Keyword [en]
ampholytes, rotofor, isoelectric focusing, bacteriocins
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-37126DOI: 10.1016/j.mimet.2007.08.002ISI: 000250426100015PubMedID: 17884211Scopus ID: 2-s2.0-34548827677OAI: oai:DiVA.org:kth-37126DiVA: diva2:432180
Available from: 2011-08-01 Created: 2011-08-01 Last updated: 2017-12-08Bibliographically approved

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