Aggregation-enhanced fluorescence in PEGylated phospholipid nanomicelles for in vivo imaging
2011 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 32, no 25, 5880-5888 p.Article in journal (Refereed) Published
We report polymeric nanomicelles doped with organic fluorophores (StCN, (Z)-2,3-bis[4-(N-4-(diphenylamino)styryl)phenyl]-acrylonitrile), which have the property of aggregation-enhanced fluorescence. The fluorescent nanomicelles have two unique features: (1) They give much brighter fluorescence emission than mono-fluorophores. (2) The nanomicelles with amphiphilic copolymers [e.g., phospholipids-PEG (polyethylene glycol)] make the encapsulated fluorophores more stable in various bio-environments and easy for further conjugation with bio-molecules. After chemical and optical characterization, these fluorescent nanomicelles are utilized as efficient optical probes for in vivo sentinel lymph node (SLN) mapping of mice. The StCN-encapsulated nanomicelles, as well as their bioconjugates with arginine-glycine-aspartic acid (RGD) peptides, are used to target subcutaneously xenografted tumors in mice, and in vivo fluorescence images demonstrate the potential to use PEGylated phospholipid nanomicelles with aggregation-enhanced fluorescence as bright nanoprobes for in vivo diagnosis of tumors.
Place, publisher, year, edition, pages
2011. Vol. 32, no 25, 5880-5888 p.
Aggregation-enhanced fluorescence, Phospholipid-PEG nanomicelles, Bright nanoprobes, In vivo imaging
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-37138DOI: 10.1016/j.biomaterials.2011.04.080ISI: 000292714700016OAI: oai:DiVA.org:kth-37138DiVA: diva2:432338