Scalable Transcriptome Preparation for Massive Parallel Sequencing
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 7, e21910- p.Article in journal (Refereed) Published
Background: The tremendous output of massive parallel sequencing technologies requires automated robust and scalable sample preparation methods to fully exploit the new sequence capacity. Methodology: In this study, a method for automated library preparation of RNA prior to massively parallel sequencing is presented. The automated protocol uses precipitation onto carboxylic acid paramagnetic beads for purification and size selection of both RNA and DNA. The automated sample preparation was compared to the standard manual sample preparation. Conclusion/Significance: The automated procedure was used to generate libraries for gene expression profiling on the Illumina HiSeq 2000 platform with the capacity of 12 samples per preparation with a significantly improved throughput compared to the standard manual preparation. The data analysis shows consistent gene expression profiles in terms of sensitivity and quantification of gene expression between the two library preparation methods.
Place, publisher, year, edition, pages
2011. Vol. 6, no 7, e21910- p.
IdentifiersURN: urn:nbn:se:kth:diva-37159DOI: 10.1371/journal.pone.0021910ISI: 000292655400026ScopusID: 2-s2.0-79960041380OAI: oai:DiVA.org:kth-37159DiVA: diva2:432390
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201108032011-08-032011-08-022015-01-15Bibliographically approved