Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
2011 (English)In: PLOS ONE, ISSN 1932-6203, Vol. 6, no 5, e19723- p.Article in journal (Refereed) Published
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
Place, publisher, year, edition, pages
2011. Vol. 6, no 5, e19723- p.
IdentifiersURN: urn:nbn:se:kth:diva-34357DOI: 10.1371/journal.pone.0019723ISI: 000290771200019ScopusID: 2-s2.0-79956258795OAI: oai:DiVA.org:kth-34357DiVA: diva2:432545
FunderFormasEU, European Research Council, LSHC-CT-2005-018704Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201108042011-08-042011-06-072011-12-06Bibliographically approved