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Rapid Screening of Complex DNA Samples by Single-Molecule Amplification and Sequencing
KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-3627-6899
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2011 (English)In: PLOS ONE, ISSN 1932-6203, Vol. 6, no 5, e19723- p.Article in journal (Refereed) Published
Abstract [en]

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.

Place, publisher, year, edition, pages
2011. Vol. 6, no 5, e19723- p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-34357DOI: 10.1371/journal.pone.0019723ISI: 000290771200019Scopus ID: 2-s2.0-79956258795OAI: oai:DiVA.org:kth-34357DiVA: diva2:432545
Funder
FormasEU, European Research Council, LSHC-CT-2005-018704Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20110804Available from: 2011-08-04 Created: 2011-06-07 Last updated: 2011-12-06Bibliographically approved

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