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A modular click approach to glycosylated polymeric beads: Design, synthesis and preliminary lectin, recognition studies
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2007 (English)In: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 40, no 21, 7513-7520 p.Article in journal (Refereed) Published
Abstract [en]

Covalent immobilization of a range of carbohydrate derivatives onto polymeric resin beads is described. Copper-catalyzed Huisgen [2 + 3] cycloaddition (often termed click chemistry) was used to graft mannose-containing azides to complementarily functionalized alkyne surfaces, namely (a) Wang resin or (b) Rasta particles consisting of a clickable alkyne polymer loose outer shell and a Wang resin inner core. For the second approach, Wang resin beads were first converted into immobilized living radical polymerization initiators with subsequent polymerization of trimethylsilanyl-protected propargyl methacrylate followed by deprotection with TBAF to yield the desired polyalkyne clickable scaffold. The appropriate (x-mannopyranoside azide was then clicked onto the bead to give a mannose functionalized Rasta resin. IR, gel-phase H-1 NMR, and elemental analysis have been used to characterize the modified resins. The binding abilities of these D-mannose-modified particles were subsequently tested using fluorescein-labeled Concanavalin A (Con A), a lectin that binds certain mannose-containing molecules. Preliminary results indicated that the novel glyco-hybrid materials presented in this work are able to efficiently recognize mannose-binding model lectins such as Con A, opening the way for their potential application in affinity chromatography, sensors, and other protein recognition/separation fields.

Place, publisher, year, edition, pages
2007. Vol. 40, no 21, 7513-7520 p.
Keyword [en]
LIVING RADICAL POLYMERIZATION, PROTEIN-CARBOHYDRATE INTERACTIONS, HUISGEN CYCLOADDITION PROCESS, CONCANAVALIN-A, GRAFT-POLYMERIZATION, STATIONARY PHASES, BLOCK-COPOLYMERS, MEMBRANE-SURFACE, TERMINAL ALKYNES, SCAVENGER RESIN
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Chemical Sciences
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URN: urn:nbn:se:kth:diva-36184DOI: 10.1021/ma071362vISI: 000250088700013Scopus ID: 2-s2.0-35548952768OAI: oai:DiVA.org:kth-36184DiVA: diva2:433840
Note
QC 20110811Available from: 2011-08-11 Created: 2011-07-08 Last updated: 2017-12-08Bibliographically approved

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