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Laboratory soft-x-ray microscope for cryotomography of biological specimens
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0002-4394-0591
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0003-2745-6289
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2011 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 36, no 14, 2728-2730 p.Article in journal (Refereed) Published
Abstract [en]

Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a lambda = 2.48nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to similar to 100nm.

Place, publisher, year, edition, pages
2011. Vol. 36, no 14, 2728-2730 p.
Keyword [en]
Computed-Tomography, Resolution
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:kth:diva-38145ISI: 000293347000043Scopus ID: 2-s2.0-79960510604OAI: oai:DiVA.org:kth-38145DiVA: diva2:436312
Note
QC 20110823Available from: 2011-08-23 Created: 2011-08-22 Last updated: 2017-12-08Bibliographically approved

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Vogt, UlrichHolmberg, AndersHertz, Hans M.

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Bertilson, Michaelvon Hofsten, OlovVogt, UlrichHolmberg, AndersChristakou, Athanasia E.Hertz, Hans M.
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Optics Letters
Atom and Molecular Physics and Optics

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