Laboratory soft-x-ray microscope for cryotomography of biological specimens
2011 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 36, no 14, 2728-2730 p.Article in journal (Refereed) Published
Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a lambda = 2.48nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to similar to 100nm.
Place, publisher, year, edition, pages
2011. Vol. 36, no 14, 2728-2730 p.
Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:kth:diva-38145ISI: 000293347000043ScopusID: 2-s2.0-79960510604OAI: oai:DiVA.org:kth-38145DiVA: diva2:436312
QC 201108232011-08-232011-08-222011-08-23Bibliographically approved