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Characterization of carboxymethyl cellulose by MALDI-TOFMS
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
2007 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology , 2007. , 32 p.
Series
Trita-CHE-Report, ISSN 1654-1081 ; 2007:28
National Category
Chemical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-38989ISBN: 978-91-7178-672-2 (print)OAI: oai:DiVA.org:kth-38989DiVA: diva2:438809
Presentation
2007-05-31, Sal E3, Osquars backe 14, KTH, Stockholm, 10:00
Note
QC 20110905Available from: 2011-09-05 Created: 2011-09-05 Last updated: 2011-09-05Bibliographically approved
List of papers
1. Improved matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of carboxymethyl cellulose
Open this publication in new window or tab >>Improved matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry of carboxymethyl cellulose
2006 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 20, no 24, 3693-3698 p.Article in journal (Refereed) Published
Abstract [en]

A refined sample preparation procedure for matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) was developed for the evaluation of the degree of substitution (DS) in partially depolymerised carboxymethyl cellulose (CMC). By adding ammonium sulphate to the sample mixture prior to the analysis, good quality mass spectra could be acquired. The usual time-consuming search for 'sweet-spots' at the crystalline rim of the MALDI target spot was also avoided. This quality improvement made it possible to investigate whether various positions on the target spot generated mass spectra in which the measured DS varied. The accuracy and reproducibility of the sample preparation procedure were tested by applying it on three commercial CMCs. The study shows that the DS values that were calculated from the spectra acquired from the centre region of the MALDI target spot were in better agreement with the DS provided by the supplier than were the values obtained from the large crystals at the target spot rim. This observation could be one reasonable explanation for the higher DS values reported in other publications. By applying our refined MALDI sample preparation procedure DS values that were in good agreement with the values provided by the manufacturer could be obtained. This indicates that MALDI-TOFMS of partially depolyrnerised CMCs can be used for an estimation of the DS as a complement to the more established methods, e.g. NMR, titrimetry, and chromatographic techniques.

Keyword
desorption, oligonucleotides, chromatography, substituents, ethers, acid
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-7104 (URN)10.1002/rcm.2786 (DOI)000242936400011 ()2-s2.0-33845565989 (Scopus ID)
Note
QC 20100720Available from: 2007-05-15 Created: 2007-05-15 Last updated: 2011-09-05Bibliographically approved
2. A New Approach for Studying Correlations between the Chemical Structure and the Rheological Properties in Carboxymethyl Cellulose
Open this publication in new window or tab >>A New Approach for Studying Correlations between the Chemical Structure and the Rheological Properties in Carboxymethyl Cellulose
2007 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 8, no 10, 3253-3257 p.Article in journal (Refereed) Published
Abstract [en]

Two model sodium carboxymethyl celluloses (CMC) with similar monomer composition but with significant differences in the viscoelastic properties, that could not be assigned to variations in the average molar mass or molar mass distribution, were investigated with respect to the fraction of nonsubstituted cellulose segments in the polymers. The CMCs were hydrolyzed by a purified highly selective endoglucanase. The average molar mass and molar mass distribution of the enzyme products, as measured by size-exclusion chromatography with online multi-angle light scattering and refractive index detection (SEC/MALS/RI), revealed that the enzyme-catalyzed hydrolysis was more effective on one of the CMCs. To investigate whether this was due to a higher fraction of nonsubstituted cellulose segments in the polymer, the concentrations of nonsubstituted enzyme products, e.g., cellotetraose and cellopentaose, were measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was concluded that the two CMCs displayed significant differences in the fraction of nonsubstituted cellulose segments. Furthermore, the CMC with the strongest attractive intermolecular interactions, according to rheometry, also contained the highest fraction of nonsubstituted cellulose segments.

Keyword
carboxymethyl cellulose, mass spectrometry, MALDI, substituent distribution, rheology, enzymatic hydrolysis
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-9433 (URN)10.1021/bm700547a (DOI)000250009900036 ()2-s2.0-35549002681 (Scopus ID)
Note
QC 20100720Available from: 2008-11-03 Created: 2008-11-03 Last updated: 2011-09-05Bibliographically approved

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