Change search
ReferencesLink to record
Permanent link

Direct link
Evaluation of the Radiocobalt-Labeled [MMA-DOTA-Cys(61)]-Z(HER2:2395)-Cys Affibody Molecule for Targeting of HER2-Expressing Tumors
2010 (English)In: Molecular Imaging and Biology, ISSN 1536-1632, E-ISSN 1860-2002, Vol. 12, no 1, 54-62 p.Article in journal (Refereed) Published
Abstract [en]

Imaging using positron emission tomography (PET) in the field of nuclear medicine is becoming increasingly important. The aim of this study was to develop a method for labeling of affibody molecules with radiocobalt for PET applications. The human epidermal growth factor receptors type 2 (HER2) binding affibody molecule DOTA-Z(2395)-C was radiolabeled with (57)Co (used as a surrogate of (55)Co). The binding specificity and cellular processing of the labeled compound was studied in vitro followed by in vivo characterization in normal and tumor-bearing mice. Furthermore, a comparative biodistribution study was performed with a (111)In-labeled counterpart. DOTA-Z(2395)-C was successfully labeled with radiocobalt with nearly quantitative yield. The compound displayed good retention on cells over time and high tumor accumulation of radioactivity in animal studies. Imaging studies showed clear visualization of HER2-positive tumors. Furthermore, the radiocobalt label provided better tumor-to-organ ratios than (111)In. Radiocobalt is a promising label for affibody molecules for future PET applications.

Place, publisher, year, edition, pages
2010. Vol. 12, no 1, 54-62 p.
Keyword [en]
PET, Cobalt, Affibody molecule, HER2, Imaging
National Category
Medical and Health Sciences Natural Sciences
URN: urn:nbn:se:kth:diva-40872DOI: 10.1007/s11307-009-0238-8ISI: 000273479300008PubMedID: 19557480OAI: diva2:442588
QC 20110922Available from: 2011-09-22 Created: 2011-09-22 Last updated: 2011-09-22Bibliographically approved
In thesis
1. Design and Evaluation of Radiolabeled Affibody Tracers for Imaging of HER2-expressing Tumors
Open this publication in new window or tab >>Design and Evaluation of Radiolabeled Affibody Tracers for Imaging of HER2-expressing Tumors
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The growing understanding of tumor biology and the identification of tumor specificgenetic and molecular alterations, such as the overexpression of human epidermal growthfactor receptor 2 (HER2), opens up for personalization of patient management using targeted therapies. However, this puts stringent demands on the diagnostic tools usedto identify patients that are likely to respond to a particular treatment. Radionuclide molecular imaging is a promising non-invasive method to visualize and characterize the expression of such targets. This thesis, based on five papers, is focused on the development of radiolabeled Affibody molecules for imaging of HER2-expression in malignant tumors. Affibody molecules, which represent a rather novel class of affinity proteins developed by combinatorial protein engineering of the protein A derived Z-domain, display manyfeatures that make them promising tracers for molecular imaging applications. The aim of the work presented here was to further develop the tracer format for improved in vivo properties and flexibility in the choice of radionuclide.

In paper I, the development of an assay that enables quantitative studies of the internalization rate and cellular processing of high affinity Affibody molecules is described. The assay was applied to a HER2-binding Affibody variant that was efficiently retained by HER2-expressing cells, although characterized by a slow internalization rate. This may have implications for the choice of label for Affibody molecules since high affinity to the target may be equally, or more, important for good imaging quality than residualizing properties of the radiolabel. In paper II, a HER2-binding Affibody molecule and the monoclonal antibody trastuzumab were labeled with positron emitting 124I, for a head-to-head in vivocomparison of the two tracer formats. The effects of tracer size and presence of an Fc region on the biodistribution profile were investigated. In paper III, a HER2-binding Affibody molecule was site-specifically labeled with radiocobalt and evaluated in vitro and in vivo.A head-to-head in vivo comparison with the well-studied 111In-labeled counterpart was performed, revealing promising potential for the cobalt-labeled molecule as a PET-tracerfor visualization of HER2. Paper IV describes the in vitro and in vivo evaluation of a panel of Affibody molecules with different C-terminal peptide-based chelators for the coordination of 99mTc. Even small changes in the C-terminal sequence had appreciable impact on the biodistribution of the Affibody molecules and by optimizing the design of the chelator, the kidney uptake of 99mTc could be significantly reduced. Finally, in paper V we describe the development of a HER2-targeting Affibody variant equipped with a Sel-tag for site-specific labeling with the short-lived positron emitter 11C. This novel Affibody tracer could be used to image HER2-expressing tumors in vivo within one hour after injection.

Taken together, Affibody molecules show great promise as targeting tracers for radionuclide molecular imaging of HER2. Careful design and optimization of the tracer protein is important and can be used to improve the biodistribution and targeting properties of Affibody molecules.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2011. xi, 79 p.
Trita-BIO-Report, ISSN 1654-2312 ; 2011:21
Affibody molecule, radionuclide molecular imaging, HER2, radiotracer, SPECT, PET, biodistribution, protein engineering, radiolabeling
National Category
Medical Biotechnology Biochemistry and Molecular Biology
urn:nbn:se:kth:diva-40890 (URN)978-91-7501-092-2 (ISBN)
Public defence
2011-10-21, FD5, AlbaNova University center, Stockholm, 10:00 (English)
QC 20110922Available from: 2011-09-22 Created: 2011-09-22 Last updated: 2011-09-22Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Wallberg, Helena
In the same journal
Molecular Imaging and Biology
Medical and Health SciencesNatural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 28 hits
ReferencesLink to record
Permanent link

Direct link