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Exploring the use of soft X-ray microscopy for imaging subcellular structures of the inner ear
KTH, Superseded Departments, Physics.
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2004 (English)In: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, Vol. 215, 203-212 p.Article in journal (Refereed) Published
Abstract [en]

The soft X-ray microscope at the Lawrence Berkeley National Laboratory was developed for visualization of biological tissue. Soft X-ray microscopy provides high-resolution visualization of hydrated, non-embedded and non-sectioned cells and is thus potentially an alternative to transmission electron microscopy. Here we show for the first time soft X-ray micrographs of structures isolated from the guinea-pig inner ear. Sensory outer hair cells and supporting pillar cells are readily visualized. In the hair cells, individual stereocilia can easily be identified within the apical hair bundle. The underlying cuticular plate is, however, too densely composed or too thick to be clearly visualized, and thus appears very dark. The cytoplasmic structures protruding from the cuticular plates as well as the fibrillar material surrounding and projecting from the cell nuclei can be seen. In the pillar cells the images reveal individual microtubule bundles. Soft X-ray images of the acellular tectorial membrane and thin two-layered Reissner's membrane display a level of resolution comparable to low-power electron microscopy.

Place, publisher, year, edition, pages
2004. Vol. 215, 203-212 p.
Keyword [en]
hair cell, inner ear, Reissner's membrane, soft X-ray microscopy, tectorial membrane
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-41207DOI: 10.1111/j.0022-2720.2004.01363.xISI: 000222897600012Scopus ID: 2-s2.0-4444225677OAI: oai:DiVA.org:kth-41207DiVA: diva2:443593
Note
QC 20110926Available from: 2011-09-26 Created: 2011-09-23 Last updated: 2017-12-08Bibliographically approved

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