Conditioned medium factors in protein-free cultures of NSO cells
2005 (English)In: Animal Cell Technology Meets Genomics / [ed] Godia, F; Fussenegger, M, 2005, 143-145 p.Conference paper (Refereed) [Artistic work]
An NS0 myeloma cell line has successfully been adapted to a protein-free medium without animal-derived components. The antibody yield in this medium is almost three-fold that in a traditional serum-medium. Kinetic studies of specific growth rate and specific product formation show that the antibody production in the serum-medium is strongly coupled to cell division while in the protein-free medium it is not. We propose that the large difference in growth and productivity profiles is due to the difference in supply and origin of growth factors. In the serum-medium proliferation is dependent on and related to factors present in the serum while in the protein-free medium the cells have adapted to an environment without serum-derived factors. Instead, the cells have started to produce their own factors through which they control their proliferation. We are currently trying to identify and characterise such conditioned medium factors and so far we have discovered at least one factor at about 20 kDa with a clear positive effect on cell growth.
Place, publisher, year, edition, pages
2005. 143-145 p.
IdentifiersURN: urn:nbn:se:kth:diva-43274ISI: 000228864200026ISBN: 1-4020-2791-5OAI: oai:DiVA.org:kth-43274DiVA: diva2:448707
18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) Location: Granada, SPAIN Date: MAY 11-14, 2003
QC 201110182011-10-182011-10-142011-10-18Bibliographically approved