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Conditioned medium factors in protein-free cultures of NSO cells
KTH, School of Biotechnology (BIO), Bioprocess Technology.
KTH, School of Biotechnology (BIO), Bioprocess Technology.
2005 (English)In: Animal Cell Technology Meets Genomics / [ed] Godia, F; Fussenegger, M, 2005, 143-145 p.Conference paper, Published paper (Refereed) [Artistic work]
Abstract [en]

An NS0 myeloma cell line has successfully been adapted to a protein-free medium without animal-derived components. The antibody yield in this medium is almost three-fold that in a traditional serum-medium. Kinetic studies of specific growth rate and specific product formation show that the antibody production in the serum-medium is strongly coupled to cell division while in the protein-free medium it is not. We propose that the large difference in growth and productivity profiles is due to the difference in supply and origin of growth factors. In the serum-medium proliferation is dependent on and related to factors present in the serum while in the protein-free medium the cells have adapted to an environment without serum-derived factors. Instead, the cells have started to produce their own factors through which they control their proliferation. We are currently trying to identify and characterise such conditioned medium factors and so far we have discovered at least one factor at about 20 kDa with a clear positive effect on cell growth.

Place, publisher, year, edition, pages
2005. 143-145 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-43274ISI: 000228864200026ISBN: 1-4020-2791-5 (print)OAI: oai:DiVA.org:kth-43274DiVA: diva2:448707
Conference
18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) Location: Granada, SPAIN Date: MAY 11-14, 2003
Note
QC 20111018Available from: 2011-10-18 Created: 2011-10-14 Last updated: 2011-10-18Bibliographically approved

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