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Identification of autocrine factors influencing proliferation in serum-free cultures of Trichoplusia ni cells
KTH, School of Biotechnology (BIO), Bioprocess Technology.
KTH, School of Biotechnology (BIO), Bioprocess Technology.
2005 (English)In: Animal Cell Technology Meets Genomics / [ed] Godia, F; Fussenegger, M, 2005, 133-135 p.Conference paper (Refereed)
Abstract [en]

The aim of this study is to understand how proliferation of Trichoplusia ni cells is regulated in serum-free cultures. The hypothesis is that T ni (Hi5) cells produce extracellular factors, which influence growth and productivity. To study this, the effect of conditioned medium (CM) on cell growth was investigated. Addition of 10 - 20% CM shortened the lag phase and increased the maximum cell density. CM was further concentrated and fractionated on a gel filtration column. Fractions which either inhibit or stimulate proliferation have been identified. These results suggest that extracellular protein factors are involved in regulation of proliferation.

Place, publisher, year, edition, pages
2005. 133-135 p.
National Category
Biological Sciences
URN: urn:nbn:se:kth:diva-43273ISI: 000228864200024ISBN: 1-4020-2791-5OAI: diva2:448714
18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) Location: Granada, SPAIN Date: MAY 11-14, 2003
QC 20111018Available from: 2011-10-18 Created: 2011-10-14 Last updated: 2011-10-18Bibliographically approved

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