Identification of autocrine factors influencing proliferation in serum-free cultures of Trichoplusia ni cells
2005 (English)In: Animal Cell Technology Meets Genomics / [ed] Godia, F; Fussenegger, M, 2005, 133-135 p.Conference paper (Refereed)
The aim of this study is to understand how proliferation of Trichoplusia ni cells is regulated in serum-free cultures. The hypothesis is that T ni (Hi5) cells produce extracellular factors, which influence growth and productivity. To study this, the effect of conditioned medium (CM) on cell growth was investigated. Addition of 10 - 20% CM shortened the lag phase and increased the maximum cell density. CM was further concentrated and fractionated on a gel filtration column. Fractions which either inhibit or stimulate proliferation have been identified. These results suggest that extracellular protein factors are involved in regulation of proliferation.
Place, publisher, year, edition, pages
2005. 133-135 p.
IdentifiersURN: urn:nbn:se:kth:diva-43273ISI: 000228864200024ISBN: 1-4020-2791-5OAI: oai:DiVA.org:kth-43273DiVA: diva2:448714
18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) Location: Granada, SPAIN Date: MAY 11-14, 2003
QC 201110182011-10-182011-10-142011-10-18Bibliographically approved