In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling
2004 (English)In: Pure and Applied Chemistry, ISSN 0033-4545, E-ISSN 1365-3075, Vol. 76, no 4, 879-888 p.Article in journal (Refereed) Published
A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications.
Place, publisher, year, edition, pages
2004. Vol. 76, no 4, 879-888 p.
IdentifiersURN: urn:nbn:se:kth:diva-43771DOI: 10.1351/pac200476040879ISI: 000222017100015ScopusID: 2-s2.0-2942674846OAI: oai:DiVA.org:kth-43771DiVA: diva2:449130
QC 201110192011-10-192011-10-182011-10-28Bibliographically approved