A novel UV-laser source for fluorescence excitation of proteins
2004 (English)In: OPTICALLY BASED BIOLOGICAL AND CHEMICAL SENSING FOR DEFENCE / [ed] Carrano, JC; Zukauskas, A, BELLINGHAM: SPIE-INT SOC OPTICAL ENGINEERING , 2004, Vol. 5617, 261-268 p.Conference paper (Refereed)
A novel, compact and robust UV laser has been developed for laser induced fluorescence spectroscopy of biomolecules in the spectral region from 290 nm to 345 nm. It was based on a frequency-doubled passively Q-switched Nd:YAG laser. emitting at 53 2 nm, which was pumping a periodically poled KTiOPO4 optical parametric oscillator with intra-cavity sum-frequency mixing in a 13130 crystal. The output was generated in two branches in the UV, 293 mn and 343 nm, with pulse widths of 1.8 ns and pulse repetition rate of 100 Hz. These wavelengths were then used for fluorescence experiments of bioagents.
Place, publisher, year, edition, pages
BELLINGHAM: SPIE-INT SOC OPTICAL ENGINEERING , 2004. Vol. 5617, 261-268 p.
, PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), ISSN 0277-786X ; 5617
diode pump solid-state laser, optical parametric oscillator, parametric devices, ultra violet generation
Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:kth:diva-43922DOI: 10.1117/12.568436ISI: 000226748300025ScopusID: 2-s2.0-17644394564ISBN: 0-8194-5570-9OAI: oai:DiVA.org:kth-43922DiVA: diva2:451444
Conference on Optically Based Biological and Chemical Sensing for Defence. London, ENGLAND. OCT 25-28, 2004
QC 201110252011-10-252011-10-192015-03-30Bibliographically approved