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PCR On A PDMS-Based Microchip With Integrated Bubble Removal
KTH, School of Electrical Engineering (EES), Microsystem Technology.
KTH, School of Electrical Engineering (EES), Microsystem Technology.ORCID iD: 0000-0002-0441-6893
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2011 (English)In: 16th International  Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011, IEEE , 2011, 2215-2218 p.Conference paper, Published paper (Refereed)
Abstract [en]

We report on the successful manufacturing and operation of the first PDMS-based PCR microreactor with continuous on-chip debubbling. A semipermeable membrane between the PCR chamber and a low-pressure chamber allows air bubbles to escape from the PCR chamber, while preventing significant water loss, thus solving one of the major problems in on-chip PCR. Moreover, a novel PDMS microfabrication process for the PCR chip is described. Finally we demonstrate successful on-chip PCR amplification.

Place, publisher, year, edition, pages
IEEE , 2011. 2215-2218 p.
Keyword [en]
Fabrication, Films, Glass, Instruments, Microfluidics, Silicon, System-on-a-chip
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-48370DOI: 10.1109/TRANSDUCERS.2011.5969303Scopus ID: 2-s2.0-80052104807ISBN: 978-1-4577-0157-3 (print)OAI: oai:DiVA.org:kth-48370DiVA: diva2:457433
Conference
16th International Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011
Note
Poster presentation. QC 20111118Available from: 2011-11-18 Created: 2011-11-17 Last updated: 2011-11-18Bibliographically approved

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Haraldsson, TommyWijngaart, Wouter van der

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Karlsson, J. MikaelHaraldsson, TommyWijngaart, Wouter van der
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