PCR On A PDMS-Based Microchip With Integrated Bubble Removal
2011 (English)In: 16th International Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011, IEEE , 2011, 2215-2218 p.Conference paper (Refereed)
We report on the successful manufacturing and operation of the first PDMS-based PCR microreactor with continuous on-chip debubbling. A semipermeable membrane between the PCR chamber and a low-pressure chamber allows air bubbles to escape from the PCR chamber, while preventing significant water loss, thus solving one of the major problems in on-chip PCR. Moreover, a novel PDMS microfabrication process for the PCR chip is described. Finally we demonstrate successful on-chip PCR amplification.
Place, publisher, year, edition, pages
IEEE , 2011. 2215-2218 p.
Fabrication, Films, Glass, Instruments, Microfluidics, Silicon, System-on-a-chip
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-48370DOI: 10.1109/TRANSDUCERS.2011.5969303ScopusID: 2-s2.0-80052104807ISBN: 978-1-4577-0157-3OAI: oai:DiVA.org:kth-48370DiVA: diva2:457433
16th International Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011
QC 201111182011-11-182011-11-172011-11-18Bibliographically approved