Electroactive porous tubular scaffolds with degradability and non-cytotoxicity for neural tissue regeneration
2011 (English)In: Acta Biomaterialia, ISSN 1742-7061, Vol. 8, no 1, 144-153 p.Article in journal (Refereed) Published
Electroactive degradable porous tubular scaffolds were fabricated from the blends of polycaprolactone and a hyperbranched degradable conducting copolymer at different feed ratios by a solution-casting/salt-leaching method. Scaning electron microscopy (SEM) and microcomputed tomography tests indicated that these scaffolds had homogeneously distributed interconnected pores on the cross-section and surface. The electrical conductivity of films with the same composition as the scaffolds was between 3.4×10(-6) and 3.1×10(-7)Scm(-1), depending on the ratio of hyperbranched degradable conducting copolymer to polycaprolactone. A hydrophilic surface with a contact angle of water about 30° was achieved by doping the films with (±)-10-camphorsulfonic acid. The mechanical properties of the films were investigated by tensile tests, and the morphology of the films was studied by SEM. The scaffolds were subjected to the WST test (a cell proliferation and cytotoxicity assay using water-soluble tetrazolium salts) with HaCaT keratinocyte cells, and the results show that these scaffolds are non-cytotoxic. These degradable electroactive tubular scaffolds are good candidates for neural tissue engineering application.
Place, publisher, year, edition, pages
2011. Vol. 8, no 1, 144-153 p.
Poly(lactide), poly(ε-caprolactone), ring-opening polymerization, carboxyl-capped aniline trimer, carboxyl-capped aniline pentamer, phenyl amino-capped aniline tetramer, coupling reaction, DCC/DMAP system, degradability, electroactivity, conductivity, macromolecular architecture, chitosan, hydrogel, block copolymer, functionalization, oxidative coupling reaction, self-assembly, toxicity, tubular porous scaffold, neural tissue engineering.
IdentifiersURN: urn:nbn:se:kth:diva-48826DOI: 10.1016/j.actbio.2011.09.027ISI: 000298763500016PubMedID: 21985870ScopusID: 2-s2.0-84855937121OAI: oai:DiVA.org:kth-48826DiVA: diva2:458642
QC 201111232011-11-232011-11-232012-04-03Bibliographically approved