Plasma Profiling Reveals Human Fibulin-1 as Candidate Marker for Renal Impairment
2011 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 10, no 11, 4925-4934 p.Article in journal (Refereed) Published
There is a need for reliable and sensitive biomarkers for renal impairments to detect early signs of kidney toxicity and to monitor progression of disease. Here, antibody suspension bead arrays were applied to profile plasma samples from patients with four types of kidney disorders: glomerulonephritis, diabetic nephropathy, obstructive uropathy, and analgesic abuse. In total, 200 clinical renal-associated cases and control plasma samples from different cohorts were profiled. Parallel plasma protein profiles were obtained using biotinylated and nonfractionated samples and a selected set of 94 proteins targeted by 129 antigen-purified polyclonal antibodies. Out of the analyzed target proteins, human fibulin-1 was detected at significantly higher levels in the glomerulonephritis patient group compared to the controls and with elevated levels in patient samples for all other renal disorders investigated. Two polyclonal antibodies and one monoclonal antibody directed toward separate, nonoverlapping epitopes showed the same trend in the discovery cohorts. A technical verification using Western blot analysis of selected patient plasma confirmed the trends toward higher abundance of the target protein in disease samples. Furthermore, a verification study was carried out in the context of glomerulonephritis using an independent case and control cohort, and this confirmed the results from the discovery cohort, suggesting that plasma levels of fibulin-1 could serve as a potential indicator to monitor kidney malfunction or kidney damage.
Place, publisher, year, edition, pages
2011. Vol. 10, no 11, 4925-4934 p.
affinity proteomics, plasma profiling, antibody microarray, kidney disorders, biomarker, fibulin-1, glomerulonephritis
IdentifiersURN: urn:nbn:se:kth:diva-51288DOI: 10.1021/pr200286cISI: 000296414700004ScopusID: 2-s2.0-80655139676OAI: oai:DiVA.org:kth-51288DiVA: diva2:463894
FunderKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201112122011-12-122011-12-122013-02-08Bibliographically approved