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Antibody-based Protein Profiling of the Human Chromosome 21
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-8993-048X
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).ORCID iD: 0000-0003-3014-5502
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101).
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2012 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 11, no 3Article in journal (Refereed) Published
Abstract [en]

A Human Proteome Project has been proposed to create a knowledgebased resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.

Place, publisher, year, edition, pages
2012. Vol. 11, no 3
National Category
Medical Biotechnology
URN: urn:nbn:se:kth:diva-51404DOI: 10.1074/mcp.M111.013458ISI: 000301296900011PubMedID: 22042635ScopusID: 2-s2.0-84857963287OAI: diva2:464103
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceKnut and Alice Wallenberg Foundation
QS 20111212Available from: 2011-12-12 Created: 2011-12-12 Last updated: 2012-04-11Bibliographically approved

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Uhlén, MathiasOksvold, PerÄlgenäs, CajsaHamsten, CarlFagerberg, LinnKlevebring, DanielLundberg, EmmaOdeberg, JacobSivertsson, Åsa
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Proteomics (closed 20130101)Science for Life Laboratory, SciLifeLab
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