Antibody-based Protein Profiling of the Human Chromosome 21
2012 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 11, no 3Article in journal (Refereed) Published
A Human Proteome Project has been proposed to create a knowledgebased resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.
Place, publisher, year, edition, pages
2012. Vol. 11, no 3
IdentifiersURN: urn:nbn:se:kth:diva-51404DOI: 10.1074/mcp.M111.013458ISI: 000301296900011PubMedID: 22042635ScopusID: 2-s2.0-84857963287OAI: oai:DiVA.org:kth-51404DiVA: diva2:464103
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscienceKnut and Alice Wallenberg Foundation
QS 201112122011-12-122011-12-122012-04-11Bibliographically approved