Assessment of antibody specificity using suspension bead arrays.
2011 (English)In: Methods in Molecular Biology, ISSN 1064-3745, Vol. 785, no Pt. 2, 183-189 p.Article in journal (Refereed) Published
With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.
Place, publisher, year, edition, pages
Springer, 2011. Vol. 785, no Pt. 2, 183-189 p.
Suspension bead array, Antibody specificity, Cross-reactivity
IdentifiersURN: urn:nbn:se:kth:diva-51523DOI: 10.1007/978-1-61779-286-1_12PubMedID: 21901600ScopusID: 2-s2.0-80054762722OAI: oai:DiVA.org:kth-51523DiVA: diva2:464415
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201112132011-12-132011-12-132011-12-13Bibliographically approved