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Assessment of antibody specificity using suspension bead arrays.
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-8141-8449
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-4657-8532
2011 (English)In: Vol. 785, no Pt. 2, 183-189 p.Article in journal (Refereed) Published
Abstract [en]

With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.

Place, publisher, year, edition, pages
Springer, 2011. Vol. 785, no Pt. 2, 183-189 p.
Keyword [en]
Suspension bead array, Antibody specificity, Cross-reactivity
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-51523DOI: 10.1007/978-1-61779-286-1_12PubMedID: 21901600Scopus ID: 2-s2.0-80054762722OAI: oai:DiVA.org:kth-51523DiVA: diva2:464415
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20111213Available from: 2011-12-13 Created: 2011-12-13 Last updated: 2017-12-08Bibliographically approved

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Schwenk, Jochen M

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