A novel plasmid addiction system for large-scale production of cyanophycin in Escherichia coli using mineral salts medium
2011 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 89, no 3, 593-604 p.Article in journal (Refereed) Published
Introduction: Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. Material and methods: In this study, we developed an anabolism-based media-dependent plasmid addiction system (PAS) to enhance plasmid stability, and we established a process based on a modified mineral salts medium yielding a CGP content of 42% (w/w) at the maximum without the addition of amino acids to the medium for the first time. This PAS is based on different lysine biosynthesis pathways and consists of two components: (1) a knockout of the chromosomal dapE disrupts the native succinylase pathway in E. coli and (2) the complementation by the plasmid-encoded artificial aminotransferase pathway mediated by the dapL gene from Synechocystis sp. PCC 6308, which allows the synthesis of the essential lysine precursor L,L-2,6-diaminopimelate. In addition, this plasmid also harbors cphAC595S, an engineered cyanophycin synthetase gene responsible for CGP production. Results: Cultivation experiments in Erlenmeyer flask and also in bioreactors in mineral salts medium without antibiotics revealed an at least 4.5-fold enhanced production of CGP in comparison to control cultivations without PAS. Discussion: Fermentation experiments with culture volume of up to 400 l yielded a maximum of 18% CGP (w/w) and a final cell density of 15.2 g CDM/l. Lactose was used constantly as an effective inducer and carbon source. Thus, we present a convenient option to produce CGP with E. coli at a technical scale without the need to add antibiotics or amino acids using the mineral salts medium designed in this study.
Place, publisher, year, edition, pages
2011. Vol. 89, no 3, 593-604 p.
Cyanophycin, DapE, DapL, Mineral salts medium, Plasmid addiction system, Succinylase pathway
Industrial Biotechnology Bioprocess Technology Microbiology
IdentifiersURN: urn:nbn:se:kth:diva-49780DOI: 10.1007/s00253-010-2899-2ISI: 000286429400012PubMedID: 20922380OAI: oai:DiVA.org:kth-49780DiVA: diva2:467133
QC 201112202011-12-192011-11-292011-12-20Bibliographically approved