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A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays
KTH, School of Biotechnology (BIO), Nano Biotechnology (closed 20130101).
KTH, School of Biotechnology (BIO), Molecular Biotechnology (closed 20130101).ORCID iD: 0000-0003-1598-8894
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-1363-5796
KTH, School of Biotechnology (BIO), Proteomics (closed 20130101). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-4657-8532
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2011 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, Vol. 12, no 11, 7748-7759 p.Article in journal (Refereed) Published
Abstract [en]

Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings.

Place, publisher, year, edition, pages
2011. Vol. 12, no 11, 7748-7759 p.
Keyword [en]
point of care, protein microarray, lateral flow
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-55264DOI: 10.3390/ijms12117748ISI: 000297696100032Scopus ID: 2-s2.0-82255181715OAI: oai:DiVA.org:kth-55264DiVA: diva2:471393
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20120102Available from: 2012-01-02 Created: 2012-01-02 Last updated: 2012-06-18Bibliographically approved

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Bass, Tarek

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