A Lateral Flow Protein Microarray for Rapid and Sensitive Antibody Assays
2011 (English)In: International Journal of Molecular Sciences, ISSN 1661-6596, Vol. 12, no 11, 7748-7759 p.Article in journal (Refereed) Published
Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein microarray device capable of sensitive (<30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nanobeads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass microarray assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings.
Place, publisher, year, edition, pages
2011. Vol. 12, no 11, 7748-7759 p.
point of care, protein microarray, lateral flow
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-55264DOI: 10.3390/ijms12117748ISI: 000297696100032ScopusID: 2-s2.0-82255181715OAI: oai:DiVA.org:kth-55264DiVA: diva2:471393
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
QC 201201022012-01-022012-01-022012-06-18Bibliographically approved