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Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
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2011 (English)In: The European Physical Journal Special Topics, ISSN 1951-6355, E-ISSN 1951-6401, Vol. 199, no 1, 181-194 p.Article in journal (Refereed) Published
Abstract [en]

The Biotin-Streptavidin complex is a widely studied system in biology and biophysics, because of its extremely strong non-covalent binding affinity. The latter is often exploited to link molecules to substrates or to one another. However, the details of the Biotin-Streptavidin binding have not been fully elucidated so far. Particularly, the role of cooperative effects in enhancing the binding affinity has not been clarified. Our long-term aim is to investigate this point by implementing two complementary approaches, fluorescence correlation spectroscopy and time-correlated single-photon counting. As both methods rely on the analysis of fluorescence signals, biotin labeled with Atto-550-dye was used. In this work, in order to get a first overview of the system, we analyzed solutions in three paradigmatic ranges of Biotin-to-Streptavidin concentration ratio. Fluorescence correlation spectroscopy measurements allowed us to extract diffusion times of free biotin and of biotin-Streptavidin complexes, and also to gain information about the dynamics of the intersystem crossing between the first excited triplet and the first excited singlet states. Time-correlated single-photon counting made it possible to derive the lifetimes of the different species in solution, as well as to deduce relevant information about the relative abundance of Streptavidin-complexed and free Biotin.

Place, publisher, year, edition, pages
2011. Vol. 199, no 1, 181-194 p.
National Category
Condensed Matter Physics
Identifiers
URN: urn:nbn:se:kth:diva-55259DOI: 10.1140/epjst/e2011-01513-1ISI: 000297814600012Scopus ID: 2-s2.0-82855164078OAI: oai:DiVA.org:kth-55259DiVA: diva2:471447
Note
QC 20120102Available from: 2012-01-02 Created: 2012-01-02 Last updated: 2017-12-08Bibliographically approved

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