Change search
ReferencesLink to record
Permanent link

Direct link
The basal ganglia output stage in the lamprey: a combined experimental and modeling study
KTH, School of Computer Science and Communication (CSC), Computational Biology, CB.ORCID iD: 0000-0002-0550-0739
2009 (English)Conference paper (Refereed)
Abstract [en]

Based on previous immunohistochemical study Robertson et al., (J Comp Neurol, 2006) believed that a lamprey homolog of the primate globus pallidus interna (GPi) exists. They found GABAergic neurons in medial pallidum projecting to optic tectum, an area homologous to the superior colliculus in mammals. Medial pallidum in lamprey also receives GABAergic projections from striatum. This study aims to investigate the electrophysiological characteristics of the putative pallidal neurons.We have used lamprey brain slices and patch recordings to characterise neurons in the medial pallidum. The glutamate blockers CNQX and AP-V have been used to test spontaneous activity dependency of excitatory synaptic input. Projecting neurons in the pallidal area are sparsely scattered and do not form an easily detectable anatomical area. To record from projecting neurons we retrogradely labelled cell somas by tracer injections. The tracer (dextran 488 coupled ) was injected in optic tectum in the anaesthetized animal 12-24 hours before the experiment. Preliminary results show both tonically active and non active neurons in the medial pallidum. A majority of the neurons in rat entopeduncular nucleus, homologous of primate GPi, are persistently spontaneously active (Nakanishi et al., Brain Res, 1991). We have found neurons with frequency adaptation and with persistent spiking when depolarising current is injected.Recordings from labelled neurons display a subpopulation of spontaneously active neurons. These neurons were found in a narrow area near the border between the diencephalon and the mesencephalon. Results from blocking glutamatergic synaptic input indicate that intrinsic neuron properties generate the spontaneous activity. We have used the experimental findings to investigate a computer model of a lamprey pallidal neuron. The model have been tuned to replicate experimental data and characteristics of lamprey pallidal neurons.We therefore conclude that neurons with pallidal properties exist in lamprey and are homologous to primate globus pallidus interna, a output stage of basal ganglia.

Place, publisher, year, edition, pages
National Category
Computer and Information Science
URN: urn:nbn:se:kth:diva-58765DOI: 10.3389/conf.neuro.11.2009.08.066OAI: diva2:473959
2nd INCF Congress of Neuroinformatics. Pilsen, Czech Republic. September 6-8 2009
QC 20120110Available from: 2012-01-08 Created: 2012-01-08 Last updated: 2012-01-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hellgren Kotaleski, Jeanette
By organisation
Computational Biology, CB
Computer and Information Science

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 12 hits
ReferencesLink to record
Permanent link

Direct link