Imaging of Vascular Smooth Muscle Cells with Soft X-Ray Spectromicroscopy
2011 (English)In: Microscopy and Microanalysis, ISSN 1431-9276, E-ISSN 1435-8115, Vol. 17, no 6, 991-1001 p.Article in journal (Refereed) Published
Using X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca L(III,II)-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined.
Place, publisher, year, edition, pages
2011. Vol. 17, no 6, 991-1001 p.
X-ray microscopy, vascular smooth muscle cells, spectromicroscopy, NEXAFS, calcium, radiation damage
Other Physics Topics
IdentifiersURN: urn:nbn:se:kth:diva-55244DOI: 10.1017/S1431927611012165ISI: 000297832300019ScopusID: 2-s2.0-82055172650OAI: oai:DiVA.org:kth-55244DiVA: diva2:474519
QC 201201092012-01-092012-01-022012-01-09Bibliographically approved