Covalent Immunoglobulin Labeling through a Photoactivable Synthetic Z Domain
2011 (English)In: Bioconjugate chemistry, ISSN 1043-1802, E-ISSN 1520-4812, Vol. 22, no 12, 2395-2403 p.Article in journal (Refereed) Published
Traditionally, labeling of antibodies has been performed by covalent conjugation to amine or carboxyl groups. These methods are efficient but suffer from nonspecificity, since all free and available amine/carboxyl groups have the possibility to react. This drawback may lead to uncontrolled levels and locations of the labeling. Hence, the labeled molecules might behave differently and, possibly, the binding site of the antibody will also be affected. In this project, we have developed a highly stringent method for labeling of antibodies by utilizing an immunoglobulin-binding domain from protein A, the Z domain. Domain Z has been synthesized with an amino acid analogue, benzoylphenylalanine, capable of forming covalent attachment to other amino acids upon UV-exposure. This feature has been used for directed labeling of immunoglobulins and subsequent use of these in different assays.
Place, publisher, year, edition, pages
2011. Vol. 22, no 12, 2395-2403 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-59002DOI: 10.1021/bc200052hISI: 000298222300006ScopusID: 2-s2.0-84555195107OAI: oai:DiVA.org:kth-59002DiVA: diva2:474583
QC 201201102012-01-092012-01-092012-05-07Bibliographically approved