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Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies
KTH, School of Biotechnology (BIO), Molecular Biotechnology.
KTH, School of Biotechnology (BIO), Molecular Biotechnology.ORCID iD: 0000-0003-1763-9073
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0002-9977-5724
KTH, School of Biotechnology (BIO), Proteomics.
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2010 (English)In: Recent Patents on Biotechnology, ISSN 1872-2083, Vol. 4, no 3, 171-182 p.Article in journal (Refereed) Published
Abstract [en]

The field of combinatorial protein engineering for generation of new affinity proteins started in the mid 80s by the development of phage display. Although phage display is a prime example of a simple yet highly efficient method, manifested by still being the standard technique 25 years later, new alternative technologies are available today. One of the more successful new display technologies is cell display. Here we review the field of cell display for directed evolution purposes, with focus on a recently developed method employing Gram-positive staphylococci as display host. Patents on the most commonly used cell display systems and on different modifications as well as specific applications of these systems are also included. General strategies for selection of new affinity proteins from cell-displayed libraries are discussed, with detailed examples mainly from studies on the staphylococcal display system. In addition, strategies for characterization of recombinant proteins on the staphylococcal cell surface, with an emphasis on an approach for epitope mapping of antibodies, are included.

Place, publisher, year, edition, pages
Bentham eBooks, 2010. Vol. 4, no 3, 171-182 p.
Keyword [en]
Affibody molecules, Antibody engineering, Bacterial display, Cell surface display, Combinatorial protein engineering, Epitope mapping, Staphylococcal surface display, Yeast display
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:kth:diva-60005DOI: 10.2174/187220810793611536PubMedID: 21171954Scopus ID: 2-s2.0-78650001663OAI: oai:DiVA.org:kth-60005DiVA: diva2:477062
Note
QC 20120113Available from: 2012-01-12 Created: 2012-01-12 Last updated: 2012-01-13Bibliographically approved

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Malm, MagdalenaUhlén, MathiasStåhl, StefanLöfblom, John

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