Change search
ReferencesLink to record
Permanent link

Direct link
A cell-relevant Microgradient Environment
KTH, School of Electrical Engineering (EES), Microsystem Technology.ORCID iD: 0000-0001-9552-4234
KTH, School of Electrical Engineering (EES), Microsystem Technology.
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
KTH, School of Biotechnology (BIO), Nano Biotechnology.
Show others and affiliations
2006 (English)In: Proceedings of the 10th International Conference in Miniaturized Systems for Chemistry and Life Sciences (µTas), 2006, 1507-1509 p.Conference paper (Refereed)
Abstract [en]

With confocal microscopy new knowledge in cell physiology is acquireddaily. However, most cell assays today are carried out either as multiwellplate assays, or in standard petridish assays. These two methods havedifferent features and foci, but they have in common the large amount ofcells submitted for treatment and imaging. In order to study only a few cellson a more detailed level[1, 2] in a relevant context, we have designed, built,and evaluated a microfluidic system. It features 1) immobilization of cells inthree dimensions, 2) transportation of cell nutrients and treatments as wellas removal of residual products, 3) an extremely stable and physiologicallyrelevant gradient of chemical concentration distribution around the cell.Previous efforts in this field by our group revealed a few very importantissues, indicating that microfabrication would be the enabling technologyfor experiments on cells in asymmetricenvironments.

Place, publisher, year, edition, pages
2006. 1507-1509 p.
Keyword [en]
gradient, cells, culture, 3D
National Category
Engineering and Technology
URN: urn:nbn:se:kth:diva-60709ScopusID: 2-s2.0-84902456033OAI: diva2:477776
Micro Total Analysis Systems (µTas) 2006. Tokyo, Japan, Nov 5-9, 2006
QC 20120116Available from: 2012-01-13 Created: 2012-01-13 Last updated: 2012-01-16Bibliographically approved

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Stemme, GöranFrisk, ThomasRydholm, SusannaAndersson, HeleneBrismar, Hjalmar
By organisation
Microsystem TechnologyCell PhysicsNano Biotechnology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 23 hits
ReferencesLink to record
Permanent link

Direct link