A cell-relevant Microgradient Environment
2006 (English)In: Proceedings of the 10th International Conference in Miniaturized Systems for Chemistry and Life Sciences (µTas), 2006, 1507-1509 p.Conference paper (Refereed)
With confocal microscopy new knowledge in cell physiology is acquireddaily. However, most cell assays today are carried out either as multiwellplate assays, or in standard petridish assays. These two methods havedifferent features and foci, but they have in common the large amount ofcells submitted for treatment and imaging. In order to study only a few cellson a more detailed level[1, 2] in a relevant context, we have designed, built,and evaluated a microfluidic system. It features 1) immobilization of cells inthree dimensions, 2) transportation of cell nutrients and treatments as wellas removal of residual products, 3) an extremely stable and physiologicallyrelevant gradient of chemical concentration distribution around the cell.Previous efforts in this field by our group revealed a few very importantissues, indicating that microfabrication would be the enabling technologyfor experiments on cells in asymmetricenvironments.
Place, publisher, year, edition, pages
2006. 1507-1509 p.
gradient, cells, culture, 3D
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-60709ScopusID: 2-s2.0-84902456033OAI: oai:DiVA.org:kth-60709DiVA: diva2:477776
Micro Total Analysis Systems (µTas) 2006. Tokyo, Japan, Nov 5-9, 2006
QC 201201162012-01-132012-01-132012-01-16Bibliographically approved