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Use of osmolytes to heat stabilise luciferase and/or apyrase
KTH, School of Biotechnology (BIO).
2002 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The present invention refers to the use of at least one osmolyte to heat stabilise at least one enzyme, such as luciferase and/or apyrase, that is used in an in-vitro bio-luminometric assay, such as a sequencing-by-synthesis reaction, especially Pyrose-quencingTM, during the enzymatic reaction. Moreover, the invention refers to a composition and a kit for use in the bioluminometric assay. Hereby, the temperature sensitive enzyme is heat stabilised, whereby the bioluminometric assay may be per-formed at a temperature that is higher and therefore more suitable for the other en-zymes of the assay.

Place, publisher, year, edition, pages
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Natural Sciences
URN: urn:nbn:se:kth:diva-61427OAI: diva2:479073
WO 2004035818

QS 2013

Available from: 2012-01-17 Created: 2012-01-17 Last updated: 2013-08-05Bibliographically approved

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Nyrén, Pål
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School of Biotechnology (BIO)
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