Estimating the variation in S phase duration from flow cytometric histograms
2008 (English)In: Mathematical Biosciences, ISSN 0025-5564, E-ISSN 1879-3134, Vol. 213, no 1, 40-49 p.Article in journal (Refereed) Published
A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.
Place, publisher, year, edition, pages
2008. Vol. 213, no 1, 40-49 p.
branching process, cell cycle progression, S phase duration, flow cytometry
Probability Theory and Statistics Cell Biology
IdentifiersURN: urn:nbn:se:kth:diva-61474DOI: 10.1016/j.mbs.2008.01.009ISI: 000256231600005OAI: oai:DiVA.org:kth-61474DiVA: diva2:479187
QC 201201182012-01-172012-01-172012-01-18Bibliographically approved