Covalent VEGF protein immobilization on resorbable polymeric surfaces
2011 (English)In: Polymers for Advanced Technologies, ISSN 1042-7147, E-ISSN 1099-1581, Vol. 22, no 12, 2368-2373 p.Article in journal (Refereed) Published
Vascular endothelial growth factor type protein (VEGF), a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L-lactic acid) (PLLA) and poly(epsilon-caprolactone) (PCL) through a three-step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non-destructive and solvent free vapor-phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using EDC/NHS chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with XPS, ATR-FTIR, UV-VIS, SEM, and ELISA.
Place, publisher, year, edition, pages
2011. Vol. 22, no 12, 2368-2373 p.
polylactide, poly(epsilon-caprolactone), vapor phase grafting, VEGF, surface
IdentifiersURN: urn:nbn:se:kth:diva-63268DOI: 10.1002/pat.1772ISI: 000298175400106OAI: oai:DiVA.org:kth-63268DiVA: diva2:483514
QC 201201232012-01-252012-01-232012-01-25Bibliographically approved