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Recombinant DNA coding for signal peptide, selective interacting polypeptide and membrane anchoring sequence
KTH, Superseded Departments, Biotechnology. (Nygren)ORCID iD: 0000-0003-4214-6991
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0001-8993-048X
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1991 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

Tripartite recombinant DNA encoding fusion proteins which comprise three sequences, i.e., a signal peptide which is operable in a Gram positive bacterium, an immunogenic polypeptide linked thereto which is not normally expressed in a Gram positive bacterium, and a cell wall spanning and a membrane anchoring sequence, as well as their use in Gram positive bacteria to express the resultant fusion protein on their surface are described. The preferred cell wall spanning and anchoring polypeptides include Staphylococcus protein A and Streptococcus protein G.

Place, publisher, year, edition, pages
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Biological Sciences
URN: urn:nbn:se:kth:diva-70221OAI: diva2:486086
US 5958736A (1999-09-28)

AU 664841 B2 (1995-12-07);  CA2103021C (2000-07-04); JP 03423712 B2 (2003-07-07)

Available from: 2012-01-30 Created: 2012-01-30 Last updated: 2015-01-22Bibliographically approved

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Nygren, Per-ÅkeUhlén, Mathias
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