Reconstruction methods in electron tomography
2008 (English)In: Mathematical Methods in Biomedical Imaging and Intensity-Modulated Radiation Therapy (IMRT) / [ed] Y. Censor, Jiang M., and Louis A. K., Springer Berlin/Heidelberg, 2008, 289-320 p.Chapter in book (Refereed)
Already in 1968 one recognized that the transmission electron micro- scope could be used in a tomographic setting as a tool for structure determination of macromolecules. However, its usage in mainstream structural biology has been limited and one reason is the devastating combination of noisy data and incomplete data problems that leads to severe ill-posedness of the inverse problem. Despite these issues, the importance of electron tomography is beginning to increase, espe- cially in drug discovery. This review begins with a brief introduction to the model for image formation, i.e. the forward operator. Next, we state the difficulties and review some of the various attempts at overcoming those in solving the inverse problem.
Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2008. 289-320 p.
Multicomponent ill-posed inverse problem, variational regularization, tolerance based entropy regularization, component-wise regularization, iterated regularization, relative entropy, electron tomography
Mathematical Analysis Structural Biology
Research subject SRA - E-Science (SeRC); SRA - Molecular Bioscience
IdentifiersURN: urn:nbn:se:kth:diva-71136ISBN: 978-88-7642-314-7OAI: oai:DiVA.org:kth-71136DiVA: diva2:486609
FunderSwedish e‐Science Research Center
QC 201201312012-01-302012-01-302012-01-31Bibliographically approved