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Adi3 is a Pdk1-interacting AGC kinase that negatively regulates plant cell death
BTI, Cornell University, USA.
BTI, Cornell University, USA.
BTI, Cornell University, USA.
BTI, Cornell University.
2006 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 25, no 1, 255-265 p.Article in journal (Refereed) Published
Abstract [en]

Bacterial speck disease in tomato is caused by Pseudomonas syringae pv. tomato. Resistance to this disease is conferred by the host Pto kinase, which recognizes P. s. pv. tomato strains that express the effector AvrPto. We report here that an AvrPto-dependent Pto-interacting protein 3 (Adi3) is a member of the AGC family of protein kinases. In mammals, AGC kinases are regulated by 3-phosphoinositide-dependent protein kinase-1 (Pdk1). We characterized tomato Pdk1 and showed that Pdk1 and Pto phosphorylate Adi3. Gene silencing of Adi3 in tomato causes MAPKKK alpha-dependent formation of necrotic lesions. Use of a chemical inhibitor of Pdk1, OSU-03012, also implicates Pdk1 and Adi3 in plant cell death regulation. Adi3 thus appears to function analogously to the mammalian AGC kinase protein kinase B/Akt by negatively regulating cell death via Pdk1 phosphorylation. We speculate that the negative regulatory function of Adi3 might be subverted by interaction with Pto/AvrPto, leading to host cell death that is associated with pathogen attack.

Place, publisher, year, edition, pages
2006. Vol. 25, no 1, 255-265 p.
Keyword [en]
Adi3, 3-phosphoinositide-dependent kinase-1, programmed cell death, protein kinase B
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-71920DOI: 10.1038/sj.emboj.7600910ISI: 000234951900024OAI: diva2:487083
QC 20120307Available from: 2012-01-31 Created: 2012-01-31 Last updated: 2012-03-07Bibliographically approved

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