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Tuning of dissolved oxygen and pH PID control parameters in large scale bioreactor by lag control
KTH, School of Biotechnology (BIO), Bioprocess Technology.ORCID iD: 0000-0002-5370-4621
KTH, School of Electrical Engineering (EES), Automatic Control. KTH, School of Electrical Engineering (EES), Centres, ACCESS Linnaeus Centre. (System Identification Group)ORCID iD: 0000-0002-9368-3079
2012 (English)In: Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), 2012, 327-330 p.Conference paper (Refereed)
Abstract [en]

A method has been developed to tune the DO and pH controller PID parameters for pilot / large scale mammalian cultivation. Our approach is to identify a model of the variable to be controlled (e.g. DO, pH) and to design several possible PID controllers based on this model. The controllers were first tested in computer simulations, followed by wet simulation and finally the best controller was tested on the real process. The approach is developed for the tuning of the DO controller of a 50 L bioreactor using microbubble continuous oxygen flow. The method, called lag control here, is based on a lead lag control design using Bode analysis where the prediction part is omitted. Experiments show that the approach results in a highly satisfactory DO control. The oxygen microbubbles were almost completely consumed before reaching the liquid surface so the oxygen flow used to maintain the DO gave an excellent indication of the cellular oxygen consumption. The control system was robust against all the perturbations, i.e. cell growth, cell bleed, addition of air-saturated fresh medium, DO set point change and a second gas sparger used to strip out the carbon dioxide. This approach was also successfully used for the tuning of a 400 L bioreactor DO controller and pH controller.

Place, publisher, year, edition, pages
2012. 327-330 p.
, ESACT Proceedings, 5 (4)
National Category
Control Engineering Bioprocess Technology
URN: urn:nbn:se:kth:diva-73457DOI: 10.1007/978-94-007-0884-6_50ISBN: 978-94-007-0883-9OAI: diva2:488919
Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT). Dublin, Ireland. June 7-10, 2009
ICT - The Next Generation

QC 20120507

Available from: 2012-02-02 Created: 2012-02-02 Last updated: 2013-04-22Bibliographically approved

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Chotteau, VéroniqueHjalmarsson, Håkan
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