Synthetic Polymer Nanoparticle-Polysaccharide Interactions: A Systematic Study
2012 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 134, no 5, 2681-2690 p.Article in journal (Refereed) Published
The interaction between synthetic polymer nanoparticles (NPs) and biomacromols. (e.g., proteins, lipids, and polysaccharides) can profoundly influence the NPs fate and function. Polysaccharides (e.g., heparin/heparin sulfate) are a key component of cell surfaces and the extracelluar matrix and play crit. roles in many biol. processes. We report a systematic investigation of the interaction between synthetic polymer nanoparticles and polysaccharides by ITC, SPR, and an anticoagulant assay to provide guidelines to engineer nanoparticles for biomedical applications. The interaction between acrylamide nanoparticles (∼30 nm) and heparin is mainly enthalpy driven with submicromolar affinity. Hydrogen bonding, ionic interactions, and dehydration of polar groups are identified to be key contributions to the affinity. It has been found that high charge d. and crosslinking of the NP can contribute to high affinity. The affinity and binding capacity of heparin can be significantly diminished by an increase in salt concn. while only slightly decreased with an increase of temp. A striking difference in binding thermodn. has been obsd. when the main component of a polymer nanoparticle is changed from acrylamide (enthalpy driven) to N-isopropylacryalmide (entropy driven). This change in thermodn. leads to different responses of these two types of polymer NPs to salt concn. and temp. Select synthetic polymer nanoparticles have also been shown to inhibit protein-heparin interactions and thus offer the potential for therapeutic applications. [on SciFinder(R)]
Place, publisher, year, edition, pages
American Chemical Society , 2012. Vol. 134, no 5, 2681-2690 p.
IdentifiersURN: urn:nbn:se:kth:diva-73751DOI: 10.1021/ja209959tISI: 000300460600041OAI: oai:DiVA.org:kth-73751DiVA: diva2:489021
CAPLUS AN 2012:40531(Journal)
QC 201305062012-02-022012-02-022013-05-06Bibliographically approved