Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A roadmap to generate renewable protein binders to the human proteome
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0002-4657-8532
KTH, School of Biotechnology (BIO), Proteomics.
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0003-1363-5796
Show others and affiliations
Number of Authors: 53
2011 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 8, no 7, 551-8 p.Article in journal (Refereed) Published
Abstract [en]

Despite the wealth of commercially available antibodies to human proteins, research is often hindered by their inconsistent validation, their poor performance and the inadequate coverage of the proteome. These issues could be addressed by systematic, genome-wide efforts to generate and validate renewable protein binders. We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked immunosorbent assay (ELISA), protein microarray and surface plasmon resonance (SPR). We also tested selected antibodies in immunoprecipitation, immunoblotting and immunofluorescence assays. Our results show that high-affinity, high-specificity renewable antibodies generated by different technologies can be produced quickly and efficiently. We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders.

Place, publisher, year, edition, pages
Nature America Inc. , 2011. Vol. 8, no 7, 551-8 p.
Keyword [en]
Immunology, HIGH-THROUGHPUT GENERATION, PHAGE DISPLAY, RECOMBINANT ANTIBODIES, AFFINITY REAGENTS
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-74487DOI: 10.1038/nmeth.1607ISI: 000292194500016PubMedID: 21572409Scopus ID: 2-s2.0-79959851876OAI: oai:DiVA.org:kth-74487DiVA: diva2:489703
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
Author count: 53. QC 20120214Available from: 2012-02-14 Created: 2012-02-03 Last updated: 2017-12-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopusPublisher's website

Authority records BETA

Schwenk, Jochen MHober, SophiaUhlén, Mathias

Search in DiVA

By author/editor
Nilsson, PeterSundberg, MårtenSjöberg, RonaldSivertsson, ÅsaSchwenk, Jochen MOttosson Takanen, JennyHober, SophiaUhlén, Mathias
By organisation
Proteomics
In the same journal
Nature Methods
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 74 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf