High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week
2009 (English)In: Biotechnology Journal, ISSN 1860-6768, Vol. 4, no 1, 51-57 p.Article in journal (Refereed) Published
The demand for high-throughput recombinant protein production has markedly increased with the increased activity in the field of proteomics. Within the Human Protein Atlas project recombinantly produced human protein fragments are used for antibody production. Here we describe how the protein expression and purification protocol has been optimized in the project to allow for han- dling of nearly 300 different proteins per week. The number of manual handling steps has been significantly reduced (from 18 to 9) and the protein purification has been completely automated.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2009. Vol. 4, no 1, 51-57 p.
High-throughput, Protein production, Proteomics
IdentifiersURN: urn:nbn:se:kth:diva-74493DOI: 10.1002/biot.200800183PubMedID: 19039781ScopusID: 2-s2.0-64549086069OAI: oai:DiVA.org:kth-74493DiVA: diva2:489710
QC 201202282012-02-282012-02-032013-11-20Bibliographically approved