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Evaluation of monospecific antibodies: A comparison study with commercial analogs using immunohistochemistry on tissue microarrays
Uppsala University. (IPG)
KTH, School of Biotechnology (BIO), Proteomics.
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0002-4657-8532
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-8993-048X
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2008 (English)In: Applied immunohistochemistry & molecular morphology (Print), ISSN 1541-2016, E-ISSN 1533-4058, Vol. 16, no 5, 493-502 p.Article in journal (Refereed) Published
Abstract [en]

Generation of monospecific antibodies (msAbs) (multiepitope) through affinity purification of polyclonal jntiscra is I plausible strategy, for high-throughput production Of affinity reagents toward large sets of proteins. These antibodies are generated using readily accessible gene Sequence information from publicly available databases. The resulting antibodies have the potential to be used in a variety of assays. probing differentially presented and altered proteins with high sensitivity and specificity. In the present study, 48 msAbs were compared with corresponding commercial analogs. Immunohistochemical staining properties were evaluated on tissue microarrays. representing various normal human tissues from 144 different individuals. MaAbs showed similar immunostaining patterns as compared with corresponding commercial analogs in 44 Out of totally 48 (92%) antibody pairs analyzed. Although only few antibody pairs showed major discrepancies. minor dissimilarities were frequently seen. Our results suggest that msAbs are reliable and valuable tools in antibody-based proteomics. enabling analysis of protein expression patterns in cells and tissues, High-throughput strategies employing such antibodies provide a consistent approach in the exploration of the human proleome.

Place, publisher, year, edition, pages
2008. Vol. 16, no 5, 493-502 p.
Keyword [en]
antibody proteomics; monospecific antibody; immunohistochemisty; tissue microarray; protein atlas
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:kth:diva-74515DOI: 10.1097/PAI.0b013e31817c645eISI: 000259648200014Scopus ID: 2-s2.0-55949089137OAI: oai:DiVA.org:kth-74515DiVA: diva2:489736
Funder
Knut and Alice Wallenberg Foundation
Note
QC 20120207Available from: 2012-02-03 Created: 2012-02-03 Last updated: 2017-12-08Bibliographically approved

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Uhlen, MathiasHober, Sophia

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